K. Bowles, PhD
Research Interests: Exercise
Teaching: Cell Biology
Dr. Bowles focuses his research on the
effects of exercise training on the heart
and coronary arteries. The primary goal
is to understand the cellular mechanisms
for functional changes observed in the heart
after endurance training. Hopefully, understanding
these changes at the cellular level will
allow for better use of exercise in preventing
Currently, research efforts in his lab
are focused on understanding the role of
ion channels, specifically K+ and Ca2+ channels
and their role in regulating coronary artery
function. We have recently discovered that
exercise training increases the contribution
of K+ channels to regulation of arterial
tone. In addition, exercise training increases
activity of L-type Ca2+ channels in coronary
arterial smooth muscle.
We are currently exploring the mechanisms
and consequences of these changes using
electrophysiology, cannulated microvessel
preparations, high speed confocal imaging,
fluorescence imaging, and biochemical techniques.
Bowles, D.K., M.H. Laughlin and M. Sturek.
Exercise training increases L-type calcium
current density in coronary smooth muscle.
Am. J. Physiol. (Heart Circ. Physiol. 44):H2159-H2169,
Bowles, DK. Adaptation of ion channels
in the microcirculation to exercise training.
Microcirc 7:25-40, 2000.
Wamhoff, B, NJ Dietz, DK Bowles and M Sturek.
Exercise training attenuates coronary smooth
muscle phenotypic modulation and nuclear
Ca2+ signaling. Am J Physiol Heart Circ
Physiol 283: H2397-H2410, 2002.
Bowles, D.K., C.L. Heaps, J. Turk, K.K.
Maddali and E. M. Price. Hypercholesterolemia
inhibits L-type calcium current in coronary
macro, not microcirculation. J. Appl. Physiol.
96: 2240-2248, 2004.
Bowles, D.K., V.K. Ganjam, K.K. Maddali,
L.J. Rubin, D.L. Tharp, J.R. Turk and C.L.
Heaps. Endogenous testosterone increases
L-type Ca2+ channel expression in porcine
coronary smooth muscle. Am. J. Physiol.
(Heart Circ. Physiol). 287: H2091-H2098,
Heaps, C.L., D.L. Tharp and D.K. Bowles.
Hypercholesterolemia abolishes voltage-dependent
K+ channel contribution to adenosine-mediated
relaxation in porcine coronary arterioles.
Am. J. Physiol. (Heart Circ. Physiol). In